C05 Intermediate ⏱ 5 Weeks 🎯 25 hours 📡 10 live sessions
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Genome Analysis & Next-Generation Sequencing

Process a whole genome — from raw FASTQ to annotated variants.

The complete NGS course. From sequencing technology principles to a working FASTQ→VCF pipeline. You will run real commands on real data and understand every step of what the world's most important genomics tool — GATK — is doing.

Enrol Now → View Curriculum
₦35,000 ≈ $42
5 WeeksDuration
25hContact Hours
10Live Sessions
5Topic Blocks
12Tools Covered

Curriculum

What you will learn

Every session is hands-on. Every topic comes with practical exercises using real biological datasets.

Week 1

Sequencing Technologies & Data Formats

  • Illumina SBS: cluster generation, reversible terminators
  • PacBio SMRT and Oxford Nanopore: long-read mechanics
  • FASTQ: Phred scores, ASCII encoding, paired-end
  • SAM/BAM: FLAG bits, CIGAR strings, header structure
Week 2

Quality Control & Read Alignment

  • FastQC: per-base quality, GC content, adapter detection
  • Trimmomatic: ILLUMINACLIP, SLIDINGWINDOW, MINLEN
  • BWA-MEM2: algorithm, read groups, alignment rates
  • Samtools: view, sort, index, flagstat, depth
Week 3

Variant Calling — GATK Best Practices

  • Picard: MarkDuplicates, alignment metrics
  • GATK HaplotypeCaller: local de novo assembly, PairHMM
  • GVCF mode: joint calling across multiple samples
  • GenotypeGVCFs: from GVCF to genotyped VCF
Week 4

Variant Filtering & Annotation

  • Hard filtering vs VQSR: when to use each approach
  • ANNOVAR: variant consequence annotation (coding, splice, regulatory)
  • ClinVar, gnomAD, dbSNP: population frequency and clinical significance
  • IGV: visualising variants, coverage, and alignments
Week 5

De Novo Assembly & Comparative Genomics

  • SPAdes: de Bruijn graph assembly for bacterial genomes
  • QUAST: N50, L50, misassemblies, completeness metrics
  • Prokka: automated prokaryotic genome annotation
  • Roary pangenome: core vs accessory genome analysis

Outcomes

What you will be able to do

Concrete, measurable skills you will take away and use immediately in your research or career.

Tools & Software

FastQCMultiQCTrimmomaticBWA-MEM2SamtoolsGATK4PicardANNOVARIGVSPAdesProkkaQUAST

Course Details

Everything included

One payment — full access to all course materials, live sessions, and community.

What's included

  • 10 live online sessions
  • 25 hours of content
  • Full pipeline scripts
  • Real GIAB benchmark data
  • GATK cheat sheet
  • Course certificate
  • Community access
  • Lifetime recordings

Who this course is for

Clinical genomics professionals · Agricultural genomics researchers · Cancer biologists · Infectious disease researchers · PhD students in genomics

Prerequisites

C01–C04 recommended. Familiarity with Linux command line strongly recommended (C06 or equivalent).

Instructor

Your instructor

N

Dr. Ashraf Akintola

PhD Biomedical Convergence Science and Technology | Bioinformatics Researcher & Educator

With close to a decade of research experience in genomics and computational biology, Dr. Noble has trained researchers across 40+ countries. His hands-on teaching philosophy has made noblekinbio one of Africa's leading bioinformatics education platforms.

What students say

Trusted by researchers worldwide

Students from 40+ countries have transformed their careers with noblekinbio courses.

"This program changed how I do research. I went from copy-pasting BLAST results to writing my own pipelines. The hands-on approach is unlike any other bioinformatics course I have taken."

CO

Chioma Okonkwo

PhD Student, University of Lagos

"Dr. Noble explains complex algorithms in a way that finally clicks. I completed C04 and C05 and immediately applied them to our malaria genomics project."

EA

Emmanuel Asante

Bioinformatics Analyst, Ghana Health Service

"I was sceptical about an online course being truly hands-on. I was wrong. Every session I was actually running code, not just watching. The Snakemake module alone was worth it."

FA

Fatima Al-Rashidi

Molecular Biology Researcher, Jordan

"C10 gave me the drug discovery computational skills I needed to transition from wet lab to computational research. I have already applied QSAR modelling in my current role."

OA

Oluwaseun Adeyemi

Pharmaceutical Scientist, Nigeria

FAQ

Common questions

How does this work with Selar? +
After clicking "Enrol Now", you will be taken to our Selar checkout page. Pay securely with card, bank transfer, or Flutterwave. Once payment is confirmed, you immediately receive access to the course content and are added to our private student community.
Do I need to attend live sessions? +
Live sessions are strongly recommended for Q&A and community, but all sessions are recorded and available in your Selar account within 24 hours. You have lifetime access to all recordings.
What if the course is not right for me? +
We offer a 7-day refund policy. If you have attended fewer than 2 sessions and completed fewer than 20% of the course, contact us at support@noblekinbio.com for a full refund.
Can I take courses in any order? +
Some courses have prerequisites (shown above). We recommend following the program sequence, but motivated students can discuss exceptions with the instructor via the community.
Is there a group discount? +
Yes — teams of 3+ receive 20% off. Contact us at enrol@noblekinbio.com with your team details.
Will I receive a certificate? +
Yes. Upon completing all assessments, you receive a digital certificate from noblekinbio.com. Completing all 13 courses earns the full Bioinformatics Certificate Program credential.
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Ready to start C05?

Process a whole genome — from raw FASTQ to annotated variants. Enrol today and get instant access to all course materials.

₦35,000 | $42 USD
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